Research Library
Peptide purity percentages explained
What a purity figure such as 98% or 99% means, how it is measured and why it should always be read with its method.
What the number means
A purity percentage typically states the proportion of the detected signal that belongs to the target compound, usually measured by HPLC at a specified wavelength.
What it does not mean
Purity by HPLC is not the same as net peptide content. A sample can show high chromatographic purity while also containing water or counter-ions, which other tests measure.
Comparing figures
Figures from different laboratories or methods are not always directly comparable. Check the method, column and detection settings stated on each report.