Research Library
Mass spectrometry explained for research buyers
What mass spectrometry measures, why it complements HPLC, and how to interpret an observed versus expected mass.
The basic principle
Mass spectrometry ionises molecules in a sample and measures their mass-to-charge ratio (m/z). The resulting spectrum shows which masses are present.
Why it complements HPLC
HPLC estimates how pure a sample is; mass spectrometry helps confirm what the main component actually is. Together they give a fuller picture of identity and purity.
Observed versus expected mass
Reports usually list the theoretical molecular weight and the observed mass. Agreement within the instrument's stated tolerance supports the identity of the compound. Larger peptides may appear as several charge states that must be deconvoluted.